Clathrin uncoating: Auxilin comes to life
نویسنده
چکیده
The DnaJ protein auxilin has been extensively studied in vitro as a cofactor for uncoating clathrin-coated vesicles by the chaperone Hsc70. Recent studies provide the first evidence that auxilin plays this role in vivo, and work on a new mammalian auxilin suggests the protein may have more complex cellular functions.
منابع مشابه
4SCIENTIFIC REVIEW Role of Auxilin and Heat Shock Protein 70kDa in Clathrin Uncoating
An interaction between auxilin and heat shock protein 70kDa (Hsc70) was initially discovered in 1995. There exists a large amount of data supporting the basis for their interaction in vitro and their function in clathrin uncoating in vivo. This review examines the key experiments in elucidating this interaction and introduces a third protein that may connect constriction or fission with hsc70/a...
متن کاملUncoating of Clathrin-Coated Vesicles in Presynaptic Terminals Roles for Hsc70 and Auxilin
We have examined the roles of Hsc70 and auxilin in the uncoating of clathrin-coated vesicles (CCVs) during neuronal endocytosis. We identified two peptides that inhibit the ability of Hsc70 and auxilin to uncoat CCVs in vitro. When injected into nerve terminals, these peptides inhibited both synaptic transmission and CCV uncoating. Mutation of a conserved HPD motif within the J domain of auxili...
متن کاملA burst of auxilin recruitment determines the onset of clathrin-coated vesicle uncoating.
Clathrin-coated pits assemble on a membrane and pinch off as coated vesicles. The released vesicles then rapidly lose their clathrin coats in a process mediated by the ATPase Hsc70, recruited by auxilin, a J-domain-containing cofactor. How is the uncoating process regulated? We find that during coat assembly small and variable amounts of auxilin are recruited transiently but that a much larger ...
متن کاملStructure of the PTEN-like region of auxilin, a detector of clathrin-coated vesicle budding.
Auxilin, a J-domain containing protein, recruits the Hsc70 uncoating ATPase to newly budded clathrin-coated vesicles. The timing of auxilin arrival determines that uncoating will commence only after the clathrin lattice has fully assembled and after membrane fission is complete. Auxilin has a region resembling PTEN, a PI3P phosphatase. We have determined the crystal structure of this region of ...
متن کاملInhibition of hsc70-catalysed clathrin uncoating by HSJ1 proteins.
The uncoating of clathrin-coated vesicles can be mediated in vitro by the 'uncoating ATPase' that has been identified as the constitutive 70 kDa heat shock protein (hsp70), hsc70. It is now established that the activity of hsp70 proteins can be regulated by another family of molecular chaperones, the DnaJ family. In this study, we have investigated the effects of DnaJ-like proteins (the human n...
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عنوان ژورنال:
- Current Biology
دوره 11 شماره
صفحات -
تاریخ انتشار 2001